Stability Indicating Rp-hplc Method for the Estimation of Zolpidem Tartrate in Bulk and Tablet Dosage Form

A novel, simple and economic reverse phase high performance liquid chromatography (RP-HPLC) Stability indicating method has been developed for the estimation of zolpidem tartrate in bulk and tablet dosage form with greater precision and accuracy. Separation was achieved on C18 column (250X4.6mm i.d.,5μm) in isocratic mode using methanol and acetonitrile in the ratio 50:50 (v/v) as mobile phase, pumped in to the column at flow rate of 1.0 mL min−1and the detection of eluent from the column was carried out using variable wavelength UV detector at 293 nm. The total run time was 10 min and the column was maintained at ambient temperature. The retention time of zolpidem tartrate was 3.467 min. The standard curves were linear over the concentration range of 10-60 μg mL−1 with r2= 0.999 and the LOD and LOQ values for zolpidem tartrate were 0.026 μg mL−1and 0.056 μg mL−1